Difference between revisions of "Part:BBa K323088:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Primers for the synthetic promoter pSYN containing a ''Bbs''I restriction site were designed. After annealing, the resulting fragment was ligated into a vector containing a double terminator ([[Part: BBa_B0015]]) as a back insert.
+
Primers for the synthetic promoter pSYN containing a ''Bbs''I restriction site were designed. After annealing, the resulting fragment was ligated into a vector containing a double terminator ([[Part:BBa_B0015]]) as a back insert.
  
 
===Source===
 
===Source===

Revision as of 19:15, 25 October 2010

In vivo testing device for protein-DNA binding: part 1 (DTER_pSYN_BbsI)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers for the synthetic promoter pSYN containing a BbsI restriction site were designed. After annealing, the resulting fragment was ligated into a vector containing a double terminator (Part:BBa_B0015) as a back insert.

Source

a

References