Difference between revisions of "Part:BBa K364315:Design"

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===Design Notes===
 
===Design Notes===
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The reconstruction of the DBD to the hinge region, LBD and Backbone will be done with RFC 25  
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The construction of the LBD and pSB1C3 backbone have been done with RFC 25.
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===Source===
 
===Source===
  
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C. elegans orphan nuclear receptor. Artifically synthetized Ligand Binding Domain of C. elegans NHR-23 placed into pSB1C3 carrier vector.
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C. elegans orphan nuclear receptor. Arteficially synthetized Ligand Binding Domain of C. elegans NHR-23 placed into pSB1C3 carrier vector.
  
 
===References===
 
===References===

Latest revision as of 19:03, 25 October 2010

NHR-23 LBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 76
    Illegal BsaI.rc site found at 382
    Illegal BsaI.rc site found at 466


Design Notes

The construction of the LBD and pSB1C3 backbone have been done with RFC 25.

Source

C. elegans orphan nuclear receptor. Arteficially synthetized Ligand Binding Domain of C. elegans NHR-23 placed into pSB1C3 carrier vector.

References

1.Kostrouchova M., Kostrouch Z., Krause M., Rall J.E. "CHR3 genomic sequence." (APR-1996) to the EMBL/GenBank/DDBJ databases

2.Kostrouchova M, Krause M, Kostrouch Z, Rall JE. "Nuclear hormone receptor CHR3 is a critical regulator of all four larval molts of the nematode Caenorhabditis elegans" [http://www.pnas.org/content/98/13/7360.longProc Natl Acad Sci U S A. 2001 Jun 19;98(13):7360-5]