Difference between revisions of "Part:BBa R0071"

 
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Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
 
Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
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===Usage and Biology===
 
===Usage and Biology===
 +
 
RhlR binds to the promoter region and activates transcription when in the presence of its autoinducer -- N-butyryl-homoserine lactone (also known as C4-HSL), which is produced by RhlI.
 
RhlR binds to the promoter region and activates transcription when in the presence of its autoinducer -- N-butyryl-homoserine lactone (also known as C4-HSL), which is produced by RhlI.
  
 
From [Medina03]:
 
From [Medina03]:
−
<i>... RhlR binds to this DNA sequence, both in vivo and in vitro, in the presence and in the absence of C4-HSL. The binding of RhlR(C4-HSL) to the las box is a necessary condition for rhlAB expression while the binding of RhlR alone to the same DNA sequence seems to repress rhlAB transcription.</i>
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&lt;i&gt;... RhlR binds to this DNA sequence, both in vivo and in vitro, in the presence and in the absence of C4-HSL. The binding of RhlR(C4-HSL) to the las box is a necessary condition for rhlAB expression while the binding of RhlR alone to the same DNA sequence seems to repress rhlAB transcription.&lt;/i&gt;
  
 
From [Medina03]:
 
From [Medina03]:
−
<i>The LasR(3-O-C12-HSL) complex can activate rhlAB promoter expression but at a considerably lower level than the RhlR(C4-HSL) complex.</i>
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&lt;i&gt;The LasR(3-O-C12-HSL) complex can activate rhlAB promoter expression but at a considerably lower level than the RhlR(C4-HSL) complex.&lt;/i&gt;
  
 
From the data in [Medina03], LasR activates rhlAB at 7.8% of the activation level achieved by RhlR.
 
From the data in [Medina03], LasR activates rhlAB at 7.8% of the activation level achieved by RhlR.
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<partinfo>BBa_R0071 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_R0071 SequenceAndFeatures</partinfo>
  
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<!-- Uncomment this to enable Functional Parameter display
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_R0071 parameters</partinfo>
 
<partinfo>BBa_R0071 parameters</partinfo>
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<!-- -->

Revision as of 00:59, 5 June 2006


Promoter (RhlR & C4-HSL regulated)

Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer.

This is the natural sequence taken from Pseudomonas aeruginosa.

Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.


Usage and Biology

RhlR binds to the promoter region and activates transcription when in the presence of its autoinducer -- N-butyryl-homoserine lactone (also known as C4-HSL), which is produced by RhlI.

From [Medina03]: <i>... RhlR binds to this DNA sequence, both in vivo and in vitro, in the presence and in the absence of C4-HSL. The binding of RhlR(C4-HSL) to the las box is a necessary condition for rhlAB expression while the binding of RhlR alone to the same DNA sequence seems to repress rhlAB transcription.</i>

From [Medina03]: <i>The LasR(3-O-C12-HSL) complex can activate rhlAB promoter expression but at a considerably lower level than the RhlR(C4-HSL) complex.</i>

From the data in [Medina03], LasR activates rhlAB at 7.8% of the activation level achieved by RhlR.

From the data in [Pearson97], the activation by LasR is about 3% of that by RhlR.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]