Difference between revisions of "Part:BBa K302029:Design"
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Revision as of 16:52, 25 October 2010
sacB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sacB sequence encodes for levansucrase enzyme of B. Subtilis. The gene has been modified by removing an ecoRI at position 201 using B. Subtilis codon bias and by changing the stop codon for the recommended double TAA.