Difference between revisions of "Part:BBa K302029:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
  
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The sacB sequence encodes for levansucrase enzyme of ''B. Subtilis''. This enzyme is natively exported from ''B.Subtilis'' cells into the extracellular enviroment where it will catalyse the conversion of sucrose in to long chain levan polymers known as levansucrose glue.
+
The sacB sequence encodes for levansucrase enzyme of ''B. Subtilis''. The gene has been modified by removing an ''ecoRI'' at position 201 using ''B. Subtilis'' codon bias and by changing the stop codon for the recommended double TAA.
  
 
===Source===
 
===Source===

Revision as of 16:52, 25 October 2010

sacB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sacB sequence encodes for levansucrase enzyme of B. Subtilis. The gene has been modified by removing an ecoRI at position 201 using B. Subtilis codon bias and by changing the stop codon for the recommended double TAA.

Source

.

References