Difference between revisions of "Part:BBa K325902"
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| − | This part contains the Genes | + | This part contains the Genes LuxE and LuxG from V.fischeri that have been codon optimised for use in E.coli. These genes are involved in the production of the substrate for the bacterial luciferase encoded by the LuxA and LuxB genes. As the proteins of the V.fischeri Lux operon are easily damaged by a high incubation temperature, transformed cells should not be grown at more than 30°C if light production is desired. |
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Latest revision as of 16:05, 25 October 2010
luxCD
V. Fischeri
(E. coli optimised)
This part contains the Genes LuxE and LuxG from V.fischeri that have been codon optimised for use in E.coli. These genes are involved in the production of the substrate for the bacterial luciferase encoded by the LuxA and LuxB genes. As the proteins of the V.fischeri Lux operon are easily damaged by a high incubation temperature, transformed cells should not be grown at more than 30°C if light production is desired.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]