Difference between revisions of "Part:BBa K364326"
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Gal4 DBD - Ecdysone receptor LBD
 
Gal4 DBD - Ecdysone receptor LBD
  
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Artificial eukaryotic TF made of Gal4 DBD (DNA Binding Domain) and D. Melanogaster nuclear receptor LBD (Ligand Binding Domain)
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Artificial eukaryotic TF made of Gal4 DBD (DNA Binding Domain) and D. Melanogaster nuclear receptor LBD (Ligand Binding Domain).
  
 
[[Image:pSB1C3-Gal4-EcR.gif|800px|thumb|center|Picture of gel electrophoresis: Gal4-EcR in pSB1C3 resulting an insert of 1142 bp. ]]
 
[[Image:pSB1C3-Gal4-EcR.gif|800px|thumb|center|Picture of gel electrophoresis: Gal4-EcR in pSB1C3 resulting an insert of 1142 bp. ]]

Revision as of 15:52, 25 October 2010

Gal4-EcR

Gal4 DBD - Ecdysone receptor LBD

Artificial eukaryotic TF made of Gal4 DBD (DNA Binding Domain) and D. Melanogaster nuclear receptor LBD (Ligand Binding Domain).

Picture of gel electrophoresis: Gal4-EcR in pSB1C3 resulting an insert of 1142 bp.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1132
    Illegal XhoI site found at 218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 137