Difference between revisions of "Part:BBa K322706"

Latest revision as of 15:41, 25 October 2010

E. coli TnaA (tryptophanase) downstream region (500bp)

This is part of a set of two biobricks (with TnaA upstream region -BBa_K322705-). It is to be used for targeting genes to the TnaA (Tryptophanase, which responsible for indole production in E. coli) with the BRIDGE recombineering method.

Any biobrick can be inserted between the upstream and downstream regions of TnaA, then inserted into the E. coli genome without leaving a marker using BRIDGE. Because it does not leave a marker, this method can be used repeatedly, and one could potentially replace the whole genome with biobricked versions.

This part is made of the 500 bases downstream of the TnaA stop codon.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K322706 short</partinfo>
-It is to be used for targeting genes to this locus in BRIDGE. In the BRIDGE, with this system, the markers are removed every time you insert a new gene, so they can be used again and again indefinitely. You could essentially replace the entire genome with new genes.
+E. coli TnaA (tryptophanase) downstream region (500bp)
+This is part of a set of two biobricks (with TnaA upstream region -BBa_K322705-). It is to be used for targeting genes to the TnaA (Tryptophanase, which responsible for indole production in E. coli) with the BRIDGE recombineering method.
+Any biobrick can be inserted between the upstream and downstream regions of TnaA, then inserted into the E. coli genome without leaving a marker using BRIDGE. Because it does not leave a marker, this method can be used repeatedly, and one could potentially replace the whole genome with biobricked versions.
+This part is made of the 500 bases downstream of the TnaA stop codon.