Difference between revisions of "Part:BBa R0065"

Revision as of 00:59, 5 June 2006

Promoter (lambda cI and luxR regulated -- hybrid)

cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR.

LuxI	cI	activity of promoter
+	+	zero
+	-	maximum
-	+	zero
-	-	leaky (no quantitative information)

Usage and Biology

Pretty good 'off' in the absence of LuxR/HSL. [jb, 5/24/04]

very leaky (no LuxR/HSL) at high (100-300) copy number. can only be slightly induced by LuxR+3OC6HSL. (jt, ut-austin; 08/11/05)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 cI repressor negatively regulates this promoter and LuxR activates its transcription.The      effect of cI is dominant over LuxR. This part is based on the LuxR and cI      repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires      the binding of two cI repressor dimers for maximal repression and contains      two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky      in the sense that 'some' transcription is seen in the absence of both cI and      LuxR.
-&nbsp;   <table width="75%" border="1">     <tr>       <td><strong>LuxI</strong></td>       <td><strong>cI</strong></td>       <td><strong>activity of promoter</strong></td>     </tr>     <tr>       <td>+</td>       <td>+</td>       <td>zero</td>     </tr>     <tr>       <td>+</td>       <td>-</td>       <td>maximum</td>     </tr>     <tr>       <td>-</td>       <td>+</td>       <td>zero</td>     </tr>     <tr>       <td>-</td>       <td>-</td>       <td>leaky (no quantitative information)</td>     </tr>   </table>
+   <table width="75%" border="1">     <tr>       <td><strong>LuxI</strong></td>       <td><strong>cI</strong></td>       <td><strong>activity of promoter</strong></td>     </tr>     <tr>       <td>+</td>       <td>+</td>       <td>zero</td>     </tr>     <tr>       <td>+</td>       <td>-</td>       <td>maximum</td>     </tr>     <tr>       <td>-</td>       <td>+</td>       <td>zero</td>     </tr>     <tr>       <td>-</td>       <td>-</td>       <td>leaky (no quantitative information)</td>     </tr>   </table>
-&nbsp;
 ===Usage and Biology===
 Pretty good 'off' in the absence of LuxR/HSL. [jb, 5/24/04]
@@ Line 17: / Line 19: @@
 <partinfo>BBa_R0065 SequenceAndFeatures</partinfo>
+<!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_R0065 parameters</partinfo>
+<!-- -->