Difference between revisions of "Part:BBa K322312:Design"

 
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<partinfo>BBa_K322312 short</partinfo>
 
<partinfo>BBa_K322312 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Notes: needed one XbaI site in luxD removed. Forward sequence is as expected. Reverse sequence does not end with TAATAA of luxE, but rather has some extra DNA, as shown, as for some reason the reverse primer was designed further downstream. The reverse sequence for clone 3 failed on this date but was repeated on 17 Sep, and read the same.
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Notes: needed one XbaI site in luxD removed. Forward sequence is as expected. Reverse sequence does not end with TAATAA of luxE, but rather has some extra DNA, as shown, as for some reason the reverse primer was designed further downstream.
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===Source===
 
===Source===

Latest revision as of 13:26, 25 October 2010

artificial operon assembled from luxCD and luxE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2410
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1332


Design Notes

Notes: needed one XbaI site in luxD removed. Forward sequence is as expected. Reverse sequence does not end with TAATAA of luxE, but rather has some extra DNA, as shown, as for some reason the reverse primer was designed further downstream.

Source

Source: artificial operon assembled from luxCD and luxE of Xenorhabdus (Photorhabdus) luminescens Hb.

References