Difference between revisions of "Part:BBa K342002:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | This part has been designed thanks to the results of the publication < | + | This part has been designed thanks to the results of the publication <a href="http://2010.igem.org/Team:INSA-Lyon/Project/References#et1">Banki et al.</a> |
The sequencing of this part is difficult because of the repetition of phasin's sequence. | The sequencing of this part is difficult because of the repetition of phasin's sequence. | ||
Revision as of 13:23, 25 October 2010
Phasin-Phasin Intein (for silver standard fusion)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1693
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1279
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part has been designed thanks to the results of the publication <a href="http://2010.igem.org/Team:INSA-Lyon/Project/References#et1">Banki et al.</a> The sequencing of this part is difficult because of the repetition of phasin's sequence.
Source
Ligation of two synthetized parts : Phasin and Phasin-Intein