Difference between revisions of "Part:BBa K422010"

 
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===Biological Background===
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The ribosome binding domain of the trigger factor trigA binds to the large subunit of the ribosome.
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===Cloning strategy===
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BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. '''Trig-A''' is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].
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Latest revision as of 11:33, 25 October 2010

trig (AarI A-part)

Biological Background

The ribosome binding domain of the trigger factor trigA binds to the large subunit of the ribosome.

Cloning strategy

BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. Trig-A is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 364
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 364
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 88