Difference between revisions of "Part:BBa R0010"
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The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bbpart>BBa_C0010</bbpart> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bbpart>BBa_R0010</bbpart> to be used as a inverter or as a detector of lactose or IPTG. | The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bbpart>BBa_C0010</bbpart> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bbpart>BBa_R0010</bbpart> to be used as a inverter or as a detector of lactose or IPTG. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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This is a direct copy of bases 0365739 through 0365540 of the E. coli K-12 MG1655 genome, Genbank NC_000913 in reverse complement form. It is the natural promoter for the LacZYA operon. | This is a direct copy of bases 0365739 through 0365540 of the E. coli K-12 MG1655 genome, Genbank NC_000913 in reverse complement form. It is the natural promoter for the LacZYA operon. | ||
It includes the tail end of the LacI gene coding region, but no promoter region for that partial gene. | It includes the tail end of the LacI gene coding region, but no promoter region for that partial gene. | ||
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<partinfo>BBa_R0010 SequenceAndFeatures</partinfo> | <partinfo>BBa_R0010 SequenceAndFeatures</partinfo> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_R0010 parameters</partinfo> | <partinfo>BBa_R0010 parameters</partinfo> | ||
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Revision as of 00:59, 5 June 2006
promoter (lacI regulated)
Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0011, etc.)
The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor BBa_C0010 which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows BBa_R0010 to be used as a inverter or as a detector of lactose or IPTG.
Usage and Biology
This is a direct copy of bases 0365739 through 0365540 of the E. coli K-12 MG1655 genome, Genbank NC_000913 in reverse complement form. It is the natural promoter for the LacZYA operon. It includes the tail end of the LacI gene coding region, but no promoter region for that partial gene.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]