Difference between revisions of "Part:BBa K367000:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | Stop codon changed to TAATAA. Codon usage adapted to Escherichia coli, using Mr. Gene's tools. Purchased from Mr. Gene. Sequenced in origin. | |
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===Source=== | ===Source=== |
Latest revision as of 09:11, 25 October 2010
prhA coding sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 658
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 724
Illegal AgeI site found at 928 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Stop codon changed to TAATAA. Codon usage adapted to Escherichia coli, using Mr. Gene's tools. Purchased from Mr. Gene. Sequenced in origin.
Source
Ralstonia solanacearum GMI1000 plasmid pGMI1000MP, complete sequence NCBI Reference Sequence: NC_003296.1