Difference between revisions of "Part:BBa K305002:Design"
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+ | Claessen, D; Rink, R; de Jong, W et al. 2003. A novel class of secreted hydrophobic proteins is involved in aerial hyphae formation in Streptomyces coelicolor by forming amyloid-like fibrils. Genes Dev 17 1714-1726 |
Revision as of 08:24, 25 October 2010
Chaplin C (chpDC) with modified signal sequence and sortase recognition motive
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 717
Illegal BamHI site found at 352 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 536
Design Notes
Ordered as construct from Mr. Gene after genomic sequence had been identified and signal sequence + sortase motive had been modified.
Source
codon optimized for B. subtilis
References
Claessen, D; Rink, R; de Jong, W et al. 2003. A novel class of secreted hydrophobic proteins is involved in aerial hyphae formation in Streptomyces coelicolor by forming amyloid-like fibrils. Genes Dev 17 1714-1726