Difference between revisions of "Part:BBa K415152:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K415152=== | ===Applications of BBa_K415152=== | ||
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| + | '''Assessing p8-GR2 incorporation into phage coat''' | ||
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| + | We transformed XL1-Blue cells with BBa_K415152, grew cells up without induction and infected with M13K07 helper phage. After 5 hours, the phage was purified, denatured, and western blotted with an anti-Myc antibody. | ||
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| + | There was no signal from the blot, indicating that inducing the part may be necessary for incorporation into the phage coat. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 06:09, 25 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K415152
Assessing p8-GR2 incorporation into phage coat
We transformed XL1-Blue cells with BBa_K415152, grew cells up without induction and infected with M13K07 helper phage. After 5 hours, the phage was purified, denatured, and western blotted with an anti-Myc antibody.
There was no signal from the blot, indicating that inducing the part may be necessary for incorporation into the phage coat.
User Reviews
UNIQ45486a343114d5b9-partinfo-00000000-QINU UNIQ45486a343114d5b9-partinfo-00000001-QINU