Difference between revisions of "Part:BBa K415151:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K415151=== | ===Applications of BBa_K415151=== | ||
| + | '''Assessing p8-GR1 incorporation into phage coat''' | ||
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| + | We transformed XL1-Blue cells with BBa_K415151, grew cells up without induction and infected with M13K07 helper phage. After 5 hours, the phage was purified, denatured, and western blotted with an anti-HA antibody. | ||
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| + | [[Image:1023westernGR1.jpg]] | ||
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| + | The purple band at 13 kD represents the p8-GR1 fusion, and is evidence of the fusion protein's incorporation into the phage coat. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 06:07, 25 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K415151
Assessing p8-GR1 incorporation into phage coat
We transformed XL1-Blue cells with BBa_K415151, grew cells up without induction and infected with M13K07 helper phage. After 5 hours, the phage was purified, denatured, and western blotted with an anti-HA antibody.
The purple band at 13 kD represents the p8-GR1 fusion, and is evidence of the fusion protein's incorporation into the phage coat.
User Reviews
UNIQ3347d9257616eeb5-partinfo-00000000-QINU UNIQ3347d9257616eeb5-partinfo-00000001-QINU