Difference between revisions of "Part:BBa K364310:Design"

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===Design Notes===
 
===Design Notes===
−
The construction of the DBD, hinge region, LBD and Backbone have been done with RFC 25
+
The construction of the LBD and Backbone have been done with RFC 25.
  
 
===Source===
 
===Source===
  
−
EcR (of the nuclear hormone receptor superfamily)
+
EcR (of the nuclear hormone receptor superfamily). Arteficially synthetized then placed into pSB1C3 carrier vector.
  
 
===References===
 
===References===
−
1.Riddiford LM, Cherbas P, Truman JW (2000). "Ecdysone receptors and their biological actions". Vitam. Horm. 60: 1–73.  
+
Riddiford LM, Cherbas P, Truman JW (2000). "Ecdysone receptors and their biological actions". Vitam. Horm. 60: 1–73.
−
2.Henrich VC (2005). "The ecdysteroid receptor". in Sarjeet S. Gill; Gilbert, Lawrence I.; Kostas Iatrou. Comprehensive molecular insect science. Amsterdam: Elsevier. pp. 243–285.
+
 +
Henrich VC (2005). "The ecdysteroid receptor". in Sarjeet S. Gill; Gilbert, Lawrence I.; Kostas Iatrou. Comprehensive molecular insect science. Amsterdam: Elsevier. pp. 243–285.
 +
 +
Koelle MR, Talbot WS, Segraves WA, Bender MT, Cherbas P, Hogness DS (October 1991). "The Drosophila EcR gene encodes an ecdysone receptor, a new member of the steroid receptor superfamily". Cell 67 (1): 59–77.

Latest revision as of 23:13, 24 October 2010

EcR LBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 683
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construction of the LBD and Backbone have been done with RFC 25.

Source

EcR (of the nuclear hormone receptor superfamily). Arteficially synthetized then placed into pSB1C3 carrier vector.

References

Riddiford LM, Cherbas P, Truman JW (2000). "Ecdysone receptors and their biological actions". Vitam. Horm. 60: 1–73.

Henrich VC (2005). "The ecdysteroid receptor". in Sarjeet S. Gill; Gilbert, Lawrence I.; Kostas Iatrou. Comprehensive molecular insect science. Amsterdam: Elsevier. pp. 243–285.

Koelle MR, Talbot WS, Segraves WA, Bender MT, Cherbas P, Hogness DS (October 1991). "The Drosophila EcR gene encodes an ecdysone receptor, a new member of the steroid receptor superfamily". Cell 67 (1): 59–77.