Difference between revisions of "Part:BBa K385004:Experience"

 
 
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===Applications of BBa_K385004===
 
===Applications of BBa_K385004===
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The N-peptide tandem repeat reading frame was fused in-frame to GFP to make a translational fusion. It was placed under control of the yeast GAL1 promoter (BBa_J63006), and transformed into yeast ''Saccharomyces cerevisiae'' in the single copy shuttle vector pRS415.
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The transformants were grown overnight in synthetic defined medium containing 2% w/v galactose, and observed using a fluorescence microscope optimised for GFP visualisation (Figure 1).
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[[Image:N25 + Gal-3.jpg|center|400 px]]
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A control culture of the same transformant was grown using glucose as the carbon source;  these conditions do not activate the GAL promoter, and as expected no GFP fluorescence was detectable (data not shown).
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Overall the results indicate that the N-peptide can be successfully expressed as a protein fusion with other standard parts.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 20:55, 24 October 2010

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Please enter how you used this part and how it worked out.

Applications of BBa_K385004

The N-peptide tandem repeat reading frame was fused in-frame to GFP to make a translational fusion. It was placed under control of the yeast GAL1 promoter (BBa_J63006), and transformed into yeast Saccharomyces cerevisiae in the single copy shuttle vector pRS415.

The transformants were grown overnight in synthetic defined medium containing 2% w/v galactose, and observed using a fluorescence microscope optimised for GFP visualisation (Figure 1).

N25 + Gal-3.jpg

A control culture of the same transformant was grown using glucose as the carbon source; these conditions do not activate the GAL promoter, and as expected no GFP fluorescence was detectable (data not shown).

Overall the results indicate that the N-peptide can be successfully expressed as a protein fusion with other standard parts.

User Reviews

UNIQc17605dc072bad3c-partinfo-00000000-QINU UNIQc17605dc072bad3c-partinfo-00000001-QINU