Difference between revisions of "Part:BBa B0011:Design"
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<partinfo>BBa_B0011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_B0011 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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===Source===
 
  
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--What is the source of this part?--
 
  
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===References===
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===Source===
  
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Derived from luxICDABEG operon terminator of Vibrio fischeri [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=nucleotide&term=AF170104 [Genbank AF170104] ].
  
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===Files===
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===References===

Revision as of 00:49, 5 June 2006


LuxICDABEG (+/-)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.



Source

Derived from luxICDABEG operon terminator of Vibrio fischeri [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=nucleotide&term=AF170104 [Genbank AF170104] ].

References