Difference between revisions of "Part:BBa K346089"

Revision as of 12:03, 24 October 2010

T7 promoter(BBa_I719005)+MerT+MerP+MerC(the facilitation module of mercury absorption)

This part conbined MerT, MerP and MerC together under the regulation of T7 promoter. MerT,merP and merC can act together to transport Hg(II) into the cytosol to activate promoters which are dependent on intracellular Hg(II). In our bioabsorbant part, we designed this part to fulfill the function that more Hg(II) will be delivered into the cytosol so that the mercury responsive promoter(PmerT) will be activated to a great extent.It then will drive downstream gene's expression with a great expression level. In this way we have made sure the proteins that were used to bind Hg(II) are expressed in large quantity.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 501
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 86
Illegal NgoMIV site found at 673
Illegal NgoMIV site found at 1094
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 79

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 In our bioabsorbant part, we designed this part to fulfill the function that more Hg(II) will be delivered into the cytosol so that the mercury responsive promoter(PmerT) will be activated to a great extent.It then will drive downstream gene's expression with a great expression level. In this way we have made sure the proteins that were used to bind Hg(II) are expressed in large quantity.
+[[Image:tpctpc.jpg]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===