Difference between revisions of "Part:BBa K313009"

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This is a device with T7 promoter to detect the function of cre recombinase.
 
This is a device with T7 promoter to detect the function of cre recombinase.
If cre recombinase expresses in the cell, the double terminator will be cut out and gfp will be expressed by T7 RNA polymerase.
+
If cre recombinase expresses in the cell, the double-terminator will be cut out irreversibly and gfp will be expressed by T7 RNA polymerase.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:50, 24 October 2010

cre recombinase assay device

This is a device with T7 promoter to detect the function of cre recombinase. If cre recombinase expresses in the cell, the double-terminator will be cut out irreversibly and gfp will be expressed by T7 RNA polymerase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 940