Difference between revisions of "Part:BBa K331030"
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<partinfo>BBa_K331030 short</partinfo> | <partinfo>BBa_K331030 short</partinfo> | ||
| − | This is <partinfo>BBa_K331022</partinfo> - which is a yellow fluorescent protein (<partinfo>E0030</partinfo> fused to a C-terminal 9x Arginine Tag (<partinfo>K249005</partinfo> preceded by a ribosomal binding site (<partinfo>B0034</partinfo>) - under the control of the tetracycline repressible promoter <partinfo>BBa_R0040</partinfo>. | + | This is <partinfo>BBa_K331022</partinfo> - which is a yellow fluorescent protein (<partinfo>E0030</partinfo>) fused to a C-terminal 9x Arginine Tag (<partinfo>K249005</partinfo>) preceded by a ribosomal binding site (<partinfo>B0034</partinfo>) - under the control of the tetracycline repressible promoter <partinfo>BBa_R0040</partinfo>. |
| − | This will be a component of a proof-of-concept part designed to show the localization of proteins into the interior of a microcompartment formed by the assembly of lumazine synthase (<partinfo>K249002</partinfo> proteins. | + | This will be a component of a proof-of-concept part designed to show the localization of proteins into the interior of a microcompartment formed by the assembly of lumazine synthase (<partinfo>K249002</partinfo>) proteins. |
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Revision as of 21:53, 23 October 2010
Tet Repressible N-terminal Arg Tagged EYFP (no terminator)
This is BBa_K331022 - which is a yellow fluorescent protein (BBa_E0030) fused to a C-terminal 9x Arginine Tag (BBa_K249005) preceded by a ribosomal binding site (BBa_B0034) - under the control of the tetracycline repressible promoter BBa_R0040.
This will be a component of a proof-of-concept part designed to show the localization of proteins into the interior of a microcompartment formed by the assembly of lumazine synthase (BBa_K249002) proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]