Difference between revisions of "Part:BBa K415300"
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| − | |FACS | + | |FACS |
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Revision as of 18:49, 23 October 2010
Collins UV Toggle - Ptrc:cI:tt:Plambda:lacI:tt
From Construction of a genetic toggle switch in E. Coli. Collins Toggle, Advanced Biotechnology, BU.
The [http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html|Collins UV Toggle] (2000) biobricked. It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.
| UV Power Modulation | |
|---|---|
| Photos | Image |
| FACS | Image |
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| The original pTSMa plasmid from the Collins paper. The parts have since been biobricked into pSB1C3. |
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1773
- 1000COMPATIBLE WITH RFC[1000]