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Revision as of 17:57, 23 October 2010

Collins UV Toggle - Ptrc:cI:tt:Plambda:lacI:tt

From Construction of a genetic toggle switch in E. Coli. Collins Toggle, Advanced Biotechnology, BU.

The [http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html|Collins UV Toggle] (2000) biobricked. It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.

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Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1773
  • 1000
    COMPATIBLE WITH RFC[1000]