Difference between revisions of "Part:BBa K364333"
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Expression vector from standard BioBrick parts, epressing Gal4 - Ecdysone receptor LBD composite part.
 
Expression vector from standard BioBrick parts, epressing Gal4 - Ecdysone receptor LBD composite part.
  
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[[Image:Team Debrecen pSB1A3-TRE-Gal4-EcR-PolyA.jpg|800px|thumb|center]]
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[[Image:Team Debrecen pSB1A3-TRE-Gal4-EcR-PolyA.jpg|800px|thumb|center|Picture of gel electrophoresis: TRE-Gal4-EcR-PolyA in pSB1A3 results a new expression vector from standard Biobrick parts. The TRE-Gal4-EcR LBD-PolyA insert is 1589 bp long, without polyA which was inserted in the last step it is 191 bp shorter.]]
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 17:40, 23 October 2010

TRE-Gal4-EcR-PolyA in PSB1A3 (expression vector)

Expression vector from standard BioBrick parts, epressing Gal4 - Ecdysone receptor LBD composite part.

Picture of gel electrophoresis: TRE-Gal4-EcR-PolyA in pSB1A3 results a new expression vector from standard Biobrick parts. The TRE-Gal4-EcR LBD-PolyA insert is 1589 bp long, without polyA which was inserted in the last step it is 191 bp shorter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1461
    Illegal XhoI site found at 547
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 466