Difference between revisions of "Part:BBa K415300"

Revision as of 17:27, 23 October 2010

Collins UV Toggle - Ptrc:cI:tt:Plambda:lacI:tt

From Construction of a genetic toggle switch in E. Coli. Collins Toggle, Advanced Biotechnology, BU.

The [http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html|Collins UV Toggle] (2000) biobricked. It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1773
1000
COMPATIBLE WITH RFC[1000]

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 From <i>Construction of a genetic toggle switch in E. Coli.</i> Collins Toggle, Advanced Biotechnology, BU.
-The [[http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html|Collins UV Toggle]] (2000) biobricked. It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.
+The [http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html|Collins UV Toggle] (2000) biobricked. It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.
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