Difference between revisions of "Part:BBa K389401:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K389401 short</partinfo>
 
<partinfo>BBa_K389401 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
arabinose promoter removed, GFP exchanged with luciferase compared to the original part <partinfo>I746370</partinfo>
+
arabinose promoter removed, GFP read-out exchanged with luciferase compared to the original part <partinfo>I746370</partinfo>
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+
−
 
+
  
 
===Source===
 
===Source===
  
−
* luciferase from pGL4.10luc2 (Promega)
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* luciferase from pGL4.10[luc2] (Promega) (<partinfo>K389004</partinfo>)
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* the rest from the parts.igem
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* sensitivity tuner part consists inter alia of
 +
** ogr activator from phage P2 (<partinfo>I746350</partinfo>) without RBS (cf. Phyiscal DNA)
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** PF promoter from phage P2 (<partinfo>I746360</partinfo>)
  
 
===References===
 
===References===
 +
[http://2007.igem.org/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]

Latest revision as of 21:56, 22 October 2010

Sensitivity tuner + luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1295
    Illegal NgoMIV site found at 2639
    Illegal NgoMIV site found at 2660
    Illegal AgeI site found at 818
    Illegal AgeI site found at 930
    Illegal AgeI site found at 1132
    Illegal AgeI site found at 2363
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2545


Design Notes

arabinose promoter removed, GFP read-out exchanged with luciferase compared to the original part BBa_I746370

Source

  • luciferase from pGL4.10[luc2] (Promega) (BBa_K389004)
  • sensitivity tuner part consists inter alia of
    • ogr activator from phage P2 (BBa_I746350) without RBS (cf. Phyiscal DNA)
    • PF promoter from phage P2 (BBa_I746360)

References

[http://2007.igem.org/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]