Difference between revisions of "Part:BBa K316007"

Revision as of 17:19, 22 October 2010

Recombinant GFP-XylE protein under Pveg promoter

This construct is designed so that the XylE activity is substantially reduced untill such a time when a TEV protease is added to the system and transcribed. TEV protease cleavable linker is positioned between the two proteins. Once the linker is cleaved, XylE is free to tetramerise and assume full activity. GFP is His tagged at the 5' end to facilitate purificaiton for in-vitro assays. This construct does not have a terminator at the end, another construct BBa_K143008 contains a double terminator BBa_B0014.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1229
Illegal NgoMIV site found at 1401
Illegal AgeI site found at 1752
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 785

Revision as of 17:18, 22 October 2010 (view source) Ks907 (Talk \| contribs) ← Older edit		Revision as of 17:19, 22 October 2010 (view source) Ks907 (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K316007 short</partinfo>		<partinfo>BBa_K316007 short</partinfo>

−	This construct is designed so that the XylE activity is substantially reduced untill such a time when a TEV protease is added to the system and transcribed. TEV protease cleavable linker is positioned between the two proteins. Once the linker is cleaved, XylE is free to tetramerise and assume full activity. GFP is His tagged at the 5' end to facilitate purificaiton for in-vitro assays. This construct does not have a terminator at the end, another construct ~~exists with a~~ <bbpart>~~BBa_B0014~~</bbpart> double terminator <bbpart>~~BBa_K143008~~</bbpart>.	+	This construct is designed so that the XylE activity is substantially reduced untill such a time when a TEV protease is added to the system and transcribed. TEV protease cleavable linker is positioned between the two proteins. Once the linker is cleaved, XylE is free to tetramerise and assume full activity. GFP is His tagged at the 5' end to facilitate purificaiton for in-vitro assays. This construct does not have a terminator at the end, another construct <bbpart>BBa_K143008</bbpart> contains a double terminator <bbpart>BBa_B0014</bbpart>.