Difference between revisions of "Part:BBa K330002:Experience"

(→‎Characterization of BBa_K330002)
(→‎Characterization of BBa_K330002)
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===Characterization of BBa_K330002===
 
===Characterization of BBa_K330002===
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'''Outlines:'''
 
'''Outlines:'''
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1. Introduction
 
1. Introduction
 +
 
2. Qualitative characterization with GUS substrate X-Gluc
 
2. Qualitative characterization with GUS substrate X-Gluc
 +
 
3. Quantitative characterization with GUS substrate 4-NPG
 
3. Quantitative characterization with GUS substrate 4-NPG
  
  
 
'''1. Introduction'''
 
'''1. Introduction'''
−
 
  
 
PBI121 containing gusA reporter gene which encoded GUS was used as the GUS producer. The promoter driving gusA was CaMV 35S, a constitutive promoter in E.coli. BioBrick BBa_I746101 which contained agrC gene but no gusA reporter gene was used as the control.
 
PBI121 containing gusA reporter gene which encoded GUS was used as the GUS producer. The promoter driving gusA was CaMV 35S, a constitutive promoter in E.coli. BioBrick BBa_I746101 which contained agrC gene but no gusA reporter gene was used as the control.
 
E.coli DH10B competent cell was used as the bacteria host for the characterization. E.coli has gusA gene as well in its genome, though, the expression of this gene is much less than that in PBI121.
 
E.coli DH10B competent cell was used as the bacteria host for the characterization. E.coli has gusA gene as well in its genome, though, the expression of this gene is much less than that in PBI121.
 +
  
 
2. Qualitative characterization with GUS substrate X-Gluc
 
2. Qualitative characterization with GUS substrate X-Gluc

Revision as of 15:00, 22 October 2010

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Applications of BBa_K330002

Promoter measurement

Characterization of BBa_K330002

Outlines:


1. Introduction

2. Qualitative characterization with GUS substrate X-Gluc

3. Quantitative characterization with GUS substrate 4-NPG


1. Introduction

PBI121 containing gusA reporter gene which encoded GUS was used as the GUS producer. The promoter driving gusA was CaMV 35S, a constitutive promoter in E.coli. BioBrick BBa_I746101 which contained agrC gene but no gusA reporter gene was used as the control. E.coli DH10B competent cell was used as the bacteria host for the characterization. E.coli has gusA gene as well in its genome, though, the expression of this gene is much less than that in PBI121.


2. Qualitative characterization with GUS substrate X-Gluc

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