Difference between revisions of "Part:BBa K411101:Design"
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PCR fusion of [[Part:BBa_E0040|GFP]] and [[Part:BBa_K411100|eIF4A]]. More details of the fusion can be found on [http://2010.igem.org/Team:NYMU-Taipei our wiki page].
 
PCR fusion of [[Part:BBa_E0040|GFP]] and [[Part:BBa_K411100|eIF4A]]. More details of the fusion can be found on [http://2010.igem.org/Team:NYMU-Taipei our wiki page].
 +
These four primers were used to fuse them together:
 +
* GFP_split_A_FP: 5' - gaattcgcggccgcttctagag atgcgtaaaggagaagaacttttc - 3' (46bp)
 +
* GFP_split_A_RP: 5' - gctgccgctgctgccgctgctgcc ttgtttgtctgccatgatgtatac - 3' (48bp)
 +
* eIF4A_split_A_FP: 5' - cggcagcagcggcagcggcagc atggagccggaaggcgtcatcga - 3' (45bp)
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* eIF4A_split_A_RP: 5' - ctgcagcggccgctactagta tca agggtctctcataaatttcttgg - 3' (47bp)
  
 
===References===
 
===References===

Revision as of 08:09, 22 October 2010

GFP_split_A + Linker + eIF4A_split_A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 741
    Illegal BglII site found at 1037
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1139


Design Notes

Source

PCR fusion of GFP and eIF4A. More details of the fusion can be found on [http://2010.igem.org/Team:NYMU-Taipei our wiki page]. These four primers were used to fuse them together:

  • GFP_split_A_FP: 5' - gaattcgcggccgcttctagag atgcgtaaaggagaagaacttttc - 3' (46bp)
  • GFP_split_A_RP: 5' - gctgccgctgctgccgctgctgcc ttgtttgtctgccatgatgtatac - 3' (48bp)
  • eIF4A_split_A_FP: 5' - cggcagcagcggcagcggcagc atggagccggaaggcgtcatcga - 3' (45bp)
  • eIF4A_split_A_RP: 5' - ctgcagcggccgctactagta tca agggtctctcataaatttcttgg - 3' (47bp)

References