Difference between revisions of "Part:BBa K314015"

Revision as of 01:32, 22 October 2010

CapD_CP-F24H

A mutated CapDCP protein aimed at increasing hydrolysis and decreasing transpeptidation.

To test BBa _K314015, it was inserted into a pET29b+ vector using Kunkel's Mutagenesis. CapD was then produced and purified as described in the [http://2010.igem.org/Team:Washington/Protocols/50mLPurificationCapD UW 2010 iGEM Team's Small Scale Protein Purification Protocol]. The purified protein was then tested for activity against CapD_CP, for a detailed description of the assay please see the [http://2010.igem.org/Team:Washington/Protocols/EnzymeAssayCapD 2010 UW iGEM wiki]. The resulting data is shown below.

Sequence and Features