Difference between revisions of "Part:BBa K314011"
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Revision as of 23:48, 21 October 2010

CapD

A protein native to Bacillus anthracis which cleaves poly-D-gamma-glutamate (PDGA) and anchors it to the bacterium's peptidoglycan

Usage and Biology

To test BBa _K314011, it was inserted into a pET29b+ vector using Kunkel's Mutagenesis. CapD was then produced and purified as described in the [http://2010.igem.org/Team:Washington/Protocols/50mLPurificationCapD UW 2010 iGEM Team's Small Scale Protein Purification Protocol]. The purified protein was then tested for activity against CapD_CP, for a detailed description of the assay please see the [http://2010.igem.org/Team:Washington/Protocols/EnzymeAssayCapD 2010 UW iGEM wiki]. The resulting data is shown below.

  • CapD MM.png
  • CapDCP MM.png

Kinetic CapD.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1540
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]