Difference between revisions of "Part:BBa K314015"

Revision as of 23:05, 21 October 2010

CapD_CP-F24H

A mutated CapDCP protein aimed at increasing hydrolysis and decreasing transpeptidation.

To test BBa _K314015, it was inserted into a pET29b+ vector using Kunkel's Mutagenesis. CapD was then produced and purified as described in the [http://2010.igem.org/Team:Washington/Protocols/50mLPurificationCapD UW 2010 iGEM Team's Small Scale Protein Purification Protocol]. The purified protein was then tested for activity against CapD_CP, for a detailed description of the assay please see the [http://2010.igem.org/Team:Washington/Protocols/EnzymeAssayCapD 2010 UW iGEM wiki]. The resulting data is shown below.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1489
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 A mutated CapDCP protein aimed at increasing hydrolysis and decreasing transpeptidation.
-To test BBa _K314015, it was inserted into a pET29b+ vector using Kunkel's Mutagenesis. CapD was then produced and purified as described in the [http://2010.igem.org/Team:Washington/Protocols/50mLPurificationCapD UW 2010 iGEM Team's Small Scale Protein Purification Protocol]. The purified protein was then tested for activity against CapD_CP, for a detailed description of the assay please see the http://2010.igem.org/Team:Washington/Protocols/EnzymeAssayCapD 2010 UW iGEM wiki].  The resulting data is shown below.
+To test BBa _K314015, it was inserted into a pET29b+ vector using Kunkel's Mutagenesis. CapD was then produced and purified as described in the [http://2010.igem.org/Team:Washington/Protocols/50mLPurificationCapD UW 2010 iGEM Team's Small Scale Protein Purification Protocol]. The purified protein was then tested for activity against CapD_CP, for a detailed description of the assay please see the [http://2010.igem.org/Team:Washington/Protocols/EnzymeAssayCapD 2010 UW iGEM wiki].  The resulting data is shown below.
 <gallery heights=400px widths=350>