Difference between revisions of "Part:BBa K314012"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K314012 short</partinfo> | <partinfo>BBa_K314012 short</partinfo> | ||
A circularly permuted CapD using a Foldit-designed linker | A circularly permuted CapD using a Foldit-designed linker | ||
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| + | To test BBa _K314012, it was inserted into a pET29b+ vector using Kunkel's Mutagenesis. CapD_CP was then produced and purified as described in the [http://2010.igem.org/Team:Washington/Protocols/50mLPurificationCapD UW 2010 iGEM Team's Small Scale Protein Purification Protocol]. The purified protein was then tested for activity, for a detailed description of the assay please see the http://2010.igem.org/Team:Washington/Protocols/EnzymeAssayCapD 2010 UW iGEM wiki]. The resulting data is shown below. | ||
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| + | <gallery heights=400px widths=350> | ||
| + | image:CapDCP_MM.png | ||
| + | </gallery> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 23:03, 21 October 2010
CapD_CP
A circularly permuted CapD using a Foldit-designed linker
To test BBa _K314012, it was inserted into a pET29b+ vector using Kunkel's Mutagenesis. CapD_CP was then produced and purified as described in the [http://2010.igem.org/Team:Washington/Protocols/50mLPurificationCapD UW 2010 iGEM Team's Small Scale Protein Purification Protocol]. The purified protein was then tested for activity, for a detailed description of the assay please see the http://2010.igem.org/Team:Washington/Protocols/EnzymeAssayCapD 2010 UW iGEM wiki]. The resulting data is shown below.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1489
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]