Difference between revisions of "Part:BBa K314200"

Revision as of 22:42, 21 October 2010

Toxin Tse2

A toxic protein originating from Pseudomonas aeruginosa that has been shown to arrest growth in both prokaryotic and eukaryotic cells when expressed intracelluarly. It is a substrate of the Pseudomonas aeruginosa type 6 secretion system. Tse2 has an immunity protein, Tsi2, that, when expressed in conjunction with Tse2 prevents cell death.

Reference showing that Tse2 is toxic, Tsi2 is the antitoxin, and that these proteins are transferred into target Gram-negative organisms via a secretion system: "A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria" [http://www.ncbi.nlm.nih.gov/pubmed/20114026]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 196
Illegal NgoMIV site found at 253
Illegal NgoMIV site found at 378
1000
COMPATIBLE WITH RFC[1000]

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 A toxic protein originating from ''Pseudomonas aeruginosa'' that has been shown to arrest growth in both prokaryotic and eukaryotic cells when expressed intracelluarly. It is a substrate of the ''Pseudomonas aeruginosa'' type 6 secretion system. Tse2 has an immunity protein, [https://parts.igem.org/wiki/index.php?title=Part:BBa_K314201 Tsi2], that, when expressed in conjunction with Tse2 prevents cell death.
-A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria [http://www.ncbi.nlm.nih.gov/pubmed/20114026]
+Reference showing that Tse2 is toxic, Tsi2 is the antitoxin, and that these proteins are transferred into target Gram-negative organisms via a secretion system: "A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria" [http://www.ncbi.nlm.nih.gov/pubmed/20114026]
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