Difference between revisions of "Part:BBa K395701:Design"

 
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===References===
 
===References===
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Nishizaki T, et al. Metabolic engineering of carotenoid biosynthesis in ''Escherichia coli'' by ordered gene assembly in Bacillus subtilis. Appl Environ Microbiol. 2007 Feb
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Seon-Kang C, et al. Characterization of bacterial β-carotene 3,3′-hydroxylases, CrtZ,
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and P450 in astaxanthin biosynthetic pathway and adonirubin
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production by gene combination in ''Escherichia coli''. Appl Microbiol Biotechnol (2006) 72
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Arunkumar K, et al. Structure of a carotenoid gene cluster from Pantoea sp. strain C1B1Y and characterization of β-carotene hydroxylase(crtZ)gene by functional complementation in ''Escherichia coli''. Carotenoid Science Vol.11 (2007)

Latest revision as of 11:01, 21 October 2010

rbs+crtZ under plac promoter for double plasmids


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

・This operon is under IPTG inducible promoter.

・I used this part with beta-carotene synthesis operon (BBa_K274210) as double plasmids in the project.



Source

Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae.

This Composite Biobrick is created by standard assembly of Part BBa_R0010, Part BBa_I742158.


References

Nishizaki T, et al. Metabolic engineering of carotenoid biosynthesis in Escherichia coli by ordered gene assembly in Bacillus subtilis. Appl Environ Microbiol. 2007 Feb

Seon-Kang C, et al. Characterization of bacterial β-carotene 3,3′-hydroxylases, CrtZ, and P450 in astaxanthin biosynthetic pathway and adonirubin production by gene combination in Escherichia coli. Appl Microbiol Biotechnol (2006) 72

Arunkumar K, et al. Structure of a carotenoid gene cluster from Pantoea sp. strain C1B1Y and characterization of β-carotene hydroxylase(crtZ)gene by functional complementation in Escherichia coli. Carotenoid Science Vol.11 (2007)