Difference between revisions of "Part:BBa K395702:Design"

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===References===
 
===References===
Tomoko Nishizaki et.al Metabolic Engineering of Carotenoid Biosynthesis in Escherichia coli
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Nishizaki T, et al. Metabolic engineering of carotenoid biosynthesis in ''Escherichia coli'' by ordered gene assembly in Bacillus subtilis. Appl Environ Microbiol. 2007 Feb
by Ordered Gene Assembly in Bacillus subtilis�Appl Environ Microbiol v.73(4); Feb 2007
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Seon-Kang C, et al. Characterization of bacterial β-carotene 3,3′-hydroxylases, CrtZ,
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and P450 in astaxanthin biosynthetic pathway and adonirubin
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production by gene combination in ''Escherichia coli''. Appl Microbiol Biotechnol (2006) 72
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Arunkumar K, et al. Structure of a carotenoid gene cluster from Pantoea sp. strain C1B1Y and characterization of β-carotene hydroxylase(crtZ)gene by functional complementation in ''Escherichia coli''. Carotenoid Science Vol.11 (2007)

Latest revision as of 10:57, 21 October 2010

rbs+crtZ under pBad promoter for double plasmids


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

・This operon is under arabinose inducible promoter.

・I used this part with beta-carotene synthesis operon (BBa_K274210) as double plasmids in the project.

Source

Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae.

This Composite Biobrick is created by standard assembly of Part BBa_I0500, Part BBa_I742158.

References

Nishizaki T, et al. Metabolic engineering of carotenoid biosynthesis in Escherichia coli by ordered gene assembly in Bacillus subtilis. Appl Environ Microbiol. 2007 Feb

Seon-Kang C, et al. Characterization of bacterial β-carotene 3,3′-hydroxylases, CrtZ, and P450 in astaxanthin biosynthetic pathway and adonirubin production by gene combination in Escherichia coli. Appl Microbiol Biotechnol (2006) 72

Arunkumar K, et al. Structure of a carotenoid gene cluster from Pantoea sp. strain C1B1Y and characterization of β-carotene hydroxylase(crtZ)gene by functional complementation in Escherichia coli. Carotenoid Science Vol.11 (2007)