Difference between revisions of "Part:BBa K314203:Design"
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<partinfo>BBa_K314203 short</partinfo> | <partinfo>BBa_K314203 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| + | Note: This part does not contain the standard biobricks scar between Lux pR and B0034 and between B0034 and Tse2/Tsi2. Instead of the standard biobrick scar at these position, this part contains the Bglbrick scar GGATCT. | ||
| − | + | This construct was originally tested in pSB3K3 with Bglbrick prefixes and suffixes. We cloned it into pSB1C3 as per the Registry's request. | |
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===Source=== | ===Source=== | ||
| − | + | ''Pseudomonas aeruginosa'' | |
===References=== | ===References=== | ||
| + | Cell Host Microbe. 2010 Jan 21;7(1):25-37 | ||
Latest revision as of 04:07, 21 October 2010
HSL inducible toxin antitoxin, Tse2 + Tsi2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1283
Illegal NgoMIV site found at 1340
Illegal NgoMIV site found at 1465
Illegal NgoMIV site found at 1795 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1004
Illegal SapI.rc site found at 1790
Design Notes
Note: This part does not contain the standard biobricks scar between Lux pR and B0034 and between B0034 and Tse2/Tsi2. Instead of the standard biobrick scar at these position, this part contains the Bglbrick scar GGATCT.
This construct was originally tested in pSB3K3 with Bglbrick prefixes and suffixes. We cloned it into pSB1C3 as per the Registry's request.
Source
Pseudomonas aeruginosa
References
Cell Host Microbe. 2010 Jan 21;7(1):25-37