Difference between revisions of "Part:BBa K314104"

 
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<partinfo>BBa_K314104 short</partinfo>
 
<partinfo>BBa_K314104 short</partinfo>
  
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Lac induced T7 protein expression insert includes f1 origin, Lac I gene and RBS
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Lac induced protein expression insert includes f1 origin K314110, Lac I generator K314111, a T7 promoter k314112, and the Elowitz standard RBS B0034.  When ligated with a protein coding sequence at the SpeI site the scar site created will create proper spacing for protein expression.
  
 
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Revision as of 02:09, 21 October 2010

T7 expression cassette

Lac induced protein expression insert includes f1 origin K314110, Lac I generator K314111, a T7 promoter k314112, and the Elowitz standard RBS B0034. When ligated with a protein coding sequence at the SpeI site the scar site created will create proper spacing for protein expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]