Difference between revisions of "Part:BBa K358010:Design"

Revision as of 10:06, 20 October 2010

anti-killer gene with GFP

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 962

Design Notes

We performed three steps of PCR to prepare this part.

First, we got S gene from lambda phage DNA: λ -Hind Ⅲ digest, and inserted into the plasmid, BBa_E0840.

Then, performed the point mutation PCR, the same to the point mutation PCR on SRRz.

Finally, we deleted a transmembrane domain, TMD1, and constructed the anti-killergene: SΔTMD1.

Source

λ -Hind Ⅲ digest (product of TaKaRa Bio) and BBa_E0240

References

R. White, T. A. T. Tran, C. A. Dankenbring, J. Deaton, and R. Young, “The N-terminal transmembrane domain of lambda S is required for holin but not antiholin function,” Journal of Bacteriology, vol. 192, no. 3, pp. 725-733, Feb. 2010.

@@ Line 6: / Line 6: @@
 ===Design Notes===
-Made point mutation and deletion PCR on S gene to prepare this part.
+We performed three steps of PCR to prepare this part.
+First, we got S gene from lambda phage DNA: λ -''Hin''d Ⅲ digest, and inserted into the plasmid, BBa_E0840.
+Then, performed the point mutation PCR, the same to the point mutation PCR on SRRz.
+Finally, we deleted a transmembrane domain, TMD1, and constructed the anti-killergene: SΔTMD1.
 ===Source===