Difference between revisions of "Part:BBa K358010:Design"

(→‎Design Notes)
(→‎References)
Line 13: Line 13:
  
 
===References===
 
===References===
 +
R. White, T. A. T. Tran, C. A. Dankenbring, J. Deaton, and R. Young, “The N-terminal transmembrane domain of lambda S is required for holin but not antiholin function,” Journal of Bacteriology, vol. 192, no. 3, pp. 725-733, Feb. 2010.

Revision as of 09:08, 20 October 2010

anti-killer gene with GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 962


Design Notes

Made point mutation and deletion PCR on S gene to prepare this part.

Source

λ -Hind Ⅲ digest (product of TaKaRa Bio) and BBa_E0240

References

R. White, T. A. T. Tran, C. A. Dankenbring, J. Deaton, and R. Young, “The N-terminal transmembrane domain of lambda S is required for holin but not antiholin function,” Journal of Bacteriology, vol. 192, no. 3, pp. 725-733, Feb. 2010.