Difference between revisions of "Part:BBa K358006:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | We performed PCR to get the lysis cassette[SRRz] and inserted before double terminator[BBa_B0015]. | |
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| + | As the template, we used λ -''Hin''d Ⅲ digest[http://catalog.takara-bio.co.jp/PDFFiles/3403_DS_j.pdf]. | ||
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| + | Forward primer: | ||
| + | Reverse primer: | ||
===Source=== | ===Source=== | ||
Revision as of 22:45, 19 October 2010
lambda lysis cassette with terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We performed PCR to get the lysis cassette[SRRz] and inserted before double terminator[BBa_B0015].
As the template, we used λ -Hind Ⅲ digest[http://catalog.takara-bio.co.jp/PDFFiles/3403_DS_j.pdf].
Forward primer:
Reverse primer:
Source
BBa_K358004 and BBa_B0015