Difference between revisions of "Part:BBa K358019"
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<partinfo>BBa_K358019 short</partinfo> | <partinfo>BBa_K358019 short</partinfo> | ||
− | Lysis cassette[SRRz] is regulated by lactose promoter. | + | Lysis cassette[SRRz] is regulated by lactose promoter. |
+ | Activating lactose promoter and expressing SRRz gene, it causes the cell lysis. So, lactose promoter must be repressed when transform this part. | ||
+ | |||
+ | To repress lactose promoter tightly, we constructed this part on low copy plasmid, pSB4K5, and transformed into KRX, not Top 10. KRX has lacI in its genome and Top 10 hasn't (see genotype[http://www.promega.com/pnotes/94/14410_27/14410_27.pdf],[http://openwetware.org/wiki/E._coli_genotypes#TOP10_.28Invitrogen.29]). | ||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 20:07, 19 October 2010
Lysis cassette regulated by lacP
Lysis cassette[SRRz] is regulated by lactose promoter. Activating lactose promoter and expressing SRRz gene, it causes the cell lysis. So, lactose promoter must be repressed when transform this part.
To repress lactose promoter tightly, we constructed this part on low copy plasmid, pSB4K5, and transformed into KRX, not Top 10. KRX has lacI in its genome and Top 10 hasn't (see genotype[http://www.promega.com/pnotes/94/14410_27/14410_27.pdf],[http://openwetware.org/wiki/E._coli_genotypes#TOP10_.28Invitrogen.29]).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]