Difference between revisions of "Part:BBa E2050:Experience"

(→‎Applications of BBa_E2050)
(→‎Applications of BBa_E2050)
Line 4: Line 4:
  
 
===Applications of BBa_E2050===
 
===Applications of BBa_E2050===
 +
<html>
 
Part:BBa_E2050:Experience
 
Part:BBa_E2050:Experience
 +
<br>
 
Aberdeen iGEM 2010 Bio-brick Experience
 
Aberdeen iGEM 2010 Bio-brick Experience
−
+
<p>
 
The yeast mOrange open reading frame (Bio-brick E2050) was tested as part of a yeast expression construct, in which E2050 was translationally fused downstream of a tandem N-peptide open reading frame (Biobrick BBa_K385004). The gene fusion was placed under control of the GAL1 promoter (BioBrick K106699). The whole construct was cloned into a yeast shuttle vector and transformed into yeast. After growth of the transformant culture in medium containing galactose to induce the promoter, expression of mOrange was assessed using flow cytometry, (excitation wavelength 488nm, fluorescence wavelength 585nm). No mOrange fluorence was detectable by this method. However, a similar, related construct, in which the mOrange sequence was replaced by GFP, did express significant quantities of green fluorescent protein (see Aberdeen¬ 2010 wiki for details). This led us to conclude the mOrange sequence was non-functional in this particular expression construct.
 
The yeast mOrange open reading frame (Bio-brick E2050) was tested as part of a yeast expression construct, in which E2050 was translationally fused downstream of a tandem N-peptide open reading frame (Biobrick BBa_K385004). The gene fusion was placed under control of the GAL1 promoter (BioBrick K106699). The whole construct was cloned into a yeast shuttle vector and transformed into yeast. After growth of the transformant culture in medium containing galactose to induce the promoter, expression of mOrange was assessed using flow cytometry, (excitation wavelength 488nm, fluorescence wavelength 585nm). No mOrange fluorence was detectable by this method. However, a similar, related construct, in which the mOrange sequence was replaced by GFP, did express significant quantities of green fluorescent protein (see Aberdeen¬ 2010 wiki for details). This led us to conclude the mOrange sequence was non-functional in this particular expression construct.
 +
</p>
 +
</html>
  
 
===User Reviews===
 
===User Reviews===

Revision as of 13:42, 16 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_E2050

Part:BBa_E2050:Experience
Aberdeen iGEM 2010 Bio-brick Experience

The yeast mOrange open reading frame (Bio-brick E2050) was tested as part of a yeast expression construct, in which E2050 was translationally fused downstream of a tandem N-peptide open reading frame (Biobrick BBa_K385004). The gene fusion was placed under control of the GAL1 promoter (BioBrick K106699). The whole construct was cloned into a yeast shuttle vector and transformed into yeast. After growth of the transformant culture in medium containing galactose to induce the promoter, expression of mOrange was assessed using flow cytometry, (excitation wavelength 488nm, fluorescence wavelength 585nm). No mOrange fluorence was detectable by this method. However, a similar, related construct, in which the mOrange sequence was replaced by GFP, did express significant quantities of green fluorescent protein (see Aberdeen¬ 2010 wiki for details). This led us to conclude the mOrange sequence was non-functional in this particular expression construct.

User Reviews

UNIQ72c79dd810e8bca8-partinfo-00000001-QINU

Antiquity

This review comes from the old result system and indicates that this part did not work in some test.

UNIQ72c79dd810e8bca8-partinfo-00000003-QINU