Difference between revisions of "Part:BBa K329004"

 
 
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<partinfo>BBa_K329004 short</partinfo>
 
<partinfo>BBa_K329004 short</partinfo>
  
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Tetracycline resistance protein (TetA(C) inner-membrane-associated protein <partinfo>BBa_J31007</partinfo>) with a Weak RBS upstream (<partinfo>BBa_B0031</partinfo>). However, overexpression of TetA(C) is detrimental to cell growth. We have designed several translational units for TetA(C) (see <partinfo>BBa_K329000</partinfo>, <partinfo>BBa_K329003</partinfo>)
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Tetracycline resistance protein (TetA(C) inner-membrane-associated protein <partinfo>BBa_J31007</partinfo>) with a Weak RBS upstream (<partinfo>BBa_B0031</partinfo>). However, overexpression of TetA(C) is detrimental to cell growth.  
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<br>We have designed several translational units for TetA(C) (see <partinfo>BBa_K329000</partinfo>, <partinfo>BBa_K329003</partinfo>).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 16:33, 12 October 2010

Weak RBS (B0031) - Tetracycline resistance protein TetA(C) (J31007)

Tetracycline resistance protein (TetA(C) inner-membrane-associated protein BBa_J31007) with a Weak RBS upstream (BBa_B0031). However, overexpression of TetA(C) is detrimental to cell growth.
We have designed several translational units for TetA(C) (see BBa_K329000, BBa_K329003).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 164
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 310
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 336
    Illegal NgoMIV site found at 704
    Illegal NgoMIV site found at 864
  • 1000
    COMPATIBLE WITH RFC[1000]