Difference between revisions of "Part:BBa K310007:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Site directed mutagenesis was performed in the coding region of ArsR due to the presence of XbaI site. This changed the coding sequence AGATCT to AGGTCT. | + | Site directed mutagenesis was performed in the coding region of ArsR at nucleotide 139 due to the presence of XbaI site. This changed the coding sequence AGATCT to AGGTCT. |
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===Source=== | ===Source=== | ||
Revision as of 00:21, 12 October 2010
ArsR repressor of the Ars Operon in E. coli K-12 MG1655
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 138
Design Notes
Site directed mutagenesis was performed in the coding region of ArsR at nucleotide 139 due to the presence of XbaI site. This changed the coding sequence AGATCT to AGGTCT.
Source
E. coli K-12 MG1655 genome