Difference between revisions of "Part:BBa K330002"

Revision as of 15:33, 10 October 2010

gusA reporter gene

The gusA reporter gene encodes beta-glucuronidase (GUS), an enzyme originated from Escherichia coli.

Originally, the GUS reporter was designed to be used in plant biology to measure the activity of a promoter. The reporter was later adopted for cell screening and promoter measurement in bacterial cells. There are three commonly used methods: histochemical assay, fluorimetrical assay and spectrophotometric assay. The GUS substrates for these assays differ: 5-bromo-4-chloro-3-indolyl glucuronide for histochemical assay, 4-methylumbelliferyl-beta-D-glucuronide for fluorimetrical assay, p-nitrophenyl β-D-glucuronide for spectrophotometric assay.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K330002 short</partinfo>
-The gusA reporter gene encodes beta-glucuronidase (GUS), an enzyme from Escherichia coli.
+The gusA reporter gene encodes beta-glucuronidase (GUS), an enzyme originated from Escherichia coli.
-Originally, the GUS reporter was designed to be used in plant biology to measure the activity of a promoter. The reporter was later adopted for cell screening and promoter measurement in bacteria. There are three often used methods: histochemical assay, fluorimetrical assay and spectrophotometric assay. The GUS substrates for these assays vary (5-bromo-4-chloro-3-indolyl glucuronide for histochemical assay,  4-methylumbelliferyl-beta-D-glucuronide for fluorimetrical assay, p-nitrophenyl &#946;-D-glucuronide for spectrophotometric assay).
+Originally, the GUS reporter was designed to be used in plant biology to measure the activity of a promoter. The reporter was later adopted for cell screening and promoter measurement in bacterial cells. There are three commonly used methods: histochemical assay, fluorimetrical assay and spectrophotometric assay. The GUS substrates for these assays differ: 5-bromo-4-chloro-3-indolyl glucuronide for histochemical assay,  4-methylumbelliferyl-beta-D-glucuronide for fluorimetrical assay, p-nitrophenyl &#946;-D-glucuronide for spectrophotometric assay.
 <!-- Add more about the biology of this part here