Difference between revisions of "Part:BBa K389011:Design"

Revision as of 12:42, 9 October 2010

VirA screening device

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

medium strong constitutive promoter,
double terminator (forward) to keep expression of kanamycin resistance by the constitutive promoter low
- double terminator BBa_B0017 instead of BBa_B0015 because of problems mentioned with BBa_B0012
kanamycin resistance for screening with plates

Source

virG gene synthesized by Mr. Gene (BBa_K389002)
vir promoter from A. tumefaciens C58 (BBa_K389003)
kanR gene made from BioBrick BBa_P1003
constitutive promoter and double terminator from parts.igem (BBa_J23110, BBa_B0017)

References

YC Jung et al. (2004) Mutants of Agrobacterium tumefaciens virG Gene That Activate Transcription of vir Promoter in Escherichia coli, Current Microbiol 49:334-340.

Revision as of 12:46, 7 October 2010 (view source) Jaretz (Talk \| contribs) (→‎Design Notes) ← Older edit		Revision as of 12:42, 9 October 2010 (view source) Jaretz (Talk \| contribs) (→‎References) Newer edit →
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	===References===		===References===
		+	YC Jung ''et al.'' (2004) Mutants of ''Agrobacterium tumefaciens virG'' Gene That Activate Transcription of ''vir'' Promoter in ''Escherichia coli'', ''Current Microbiol'' 49:334-340.