Difference between revisions of "Part:BBa K318502:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | We wanted to test what IPTG induction levels were | + | We wanted to test what IPTG induction levels were appropriate to protect our cells from an acidic environment of pH=2 before placing our devices under constitute control with appropriate RBS's. |
===Source=== | ===Source=== |
Revision as of 23:06, 8 October 2010
lacI pL + RBS + RcsB + RBS + RcsA + TT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We wanted to test what IPTG induction levels were appropriate to protect our cells from an acidic environment of pH=2 before placing our devices under constitute control with appropriate RBS's.
Source
The RcsA gene we used was originally submitted by CalTech 2008 <part info>BBa_K137113</part info> and the RcsB gene we used was originally submitted by Imperial 2009 <part info>BBa_K200000</part info>.